To develop biocompatible composite microspheres for novel hemostatic use, we designed and prepared a novel biomaterial, composite microspheres consisting of carboxymethyl chitosan, sodium alginate, and collagen (CSCM). The ultra-structure of CSCM was investigated by scanning electron microscopy assay. In hemostatic function experiment, it was found that CSCM could facilitate platelet adherence, platelet aggregation, and platelet activation in vitro. Besides, the maximum swelling of CSCM submerged in PBS for 50 min was over 300% of that exhibited by commercial hemostatic compound microporous polysaccharide haemostatic powder (CMPHP). In addition, CSCM exhibited good biodegradability and non-cytotoxicity. These results demonstrated that CSCM may be useful in platelet plug formation, and this study would provide important information for further research on hemostasis experiment in vivo.

Hyperbranched poly (amino amine) s (HPAMAMs), synthesized by Michael addition of 1-(2-aminoethyl) piperazine (AEPZ) and methyl acrylate (MA), can emit blue fluorescence under excitation wavelength. However, the relatively weak fluorescence of HPAMAMs is still an obstacle for its practical applications. Gold is one of the most frequently used metals for fluorescence enhancement. In this research, the influence of gold nanoparticles on fluorescence property of HPAMAMs was investigated by fluorometry. It was found that gold nanoparticles (GNPs) with smaller size (< 5 nm) presented greatly enhanced emission. The GNPs with larger size (~10 nm in diameter) and definite surface plasmon absorption can quench the fluorescence of HPAMAMs.