Introduction: Married immigrant women experience cultural conflicts due to different values, and customs. This study examined the factors associated with the acculturation and acculturative stress of Asian immigrant women in South Korea. Method: A cross-sectional descriptive study was performed. A total of 142 participants were recruited from Incheon Multicultural Centers in 2016 using a convenience sampling method. Acculturation and acculturative stress were measured by Suinn–Lew Self-Identity Acculturation Scale and Social Attitudinal Familial Environment. The data were analyzed using analysis of variance and multiple regression analysis. Results: Time since immigration was negatively associated with the level of acculturation, while alcohol consumption ( p < .050). Some demographic variables (age, religion, anxiety, and time since immigration) were associated with acculturative stress ( p < .050). Discussion: These findings can help health care providers to increase their awareness and be more sensitive when providing services to these women.