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A simple yet rapid high performance liquid chromatography (HPLC) technique has been developed to separate and quantitate mono-and di-saccharides and sorbitol in foods. The technique differs from previously described HPLC methods in that (a) a pair of HPLC columns are joined in tandem for better resolution of the glucose-sorbitol peak, and (2) a ternary solvent system (acetonitrile/water/ethanol) with isocratic elution is used to increase resolution and decrease total analysistime. Elution order is fructose, glucose, sorbitol, sucrose, maltose, and lactose, all within 22 mm.