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A thin-layer chromatographic (TLC) method for determining deoxynivalenol, fusarenon-X, and nivalenol in barley, corn, and wheat has been developed. The toxins were extracted witth acetonitrile-watermethanol and purified by lead acetate precipitation, filtration through an alumina-charcoal-Celite column, and separation on an octadecylsilane column. TLC on an aluminum chloride impregnated silica gel plate was used for quantitation. After the plate was developed with two different compositions of chloroform-acetone-2-propanol, it was heated to produce blue fluorescent spots, which were quantitated fluorodensitometrically. Average recovery of the three toxins at levels of 100 and 200 ng/g was 83%, and the limit of determination was 50 ng/g. Eight test samples naturally contaminated with nivalenol and/or deoxynivalenol were also analyzed. The identity of the three toxins was confirmed after trimethylsilyl derivative formation and gas chromatographic separation followed by matrix isolation/Fourier transform infrared spectrometric analysis.

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