The allelopathic potential of medicinal plant Filipendula vulgaris Moench, originated from two natural habitats: calcareous grasslands and humid meadows, was investigated using radish (Raphanus sativus L. var. radicula Pers.) as a model plant. Suppression influence of aqueous extracts at concentrations: 1%, 5%, 10%, obtained separately from under and aboveground parts of the species, was studied testing germination capacity, biomass and biometry of seedling, and electrolyte leakage. Additionally, two cultivars of the radish were used to test the sensitivity of frequently used plant in the laboratory tests. This study revealed the inhibiting effect of high concentration aqueous extracts from F. vulgaris organs on the germination of radish. It was significant for seed vigour index, speed emergence, germination stress tolerance index, and mean germination time. However, the stimulating effect of 1% of aqueous extracts from dropwort roots and shoots on the germination indexes was documented. Similarly, low concentration extracts had a positive effect on seedling elongation which was visible in biometric analyses and inhibition percentage index but it was not so obvious in the biomass analyses. In general, the aboveground extracts from dropwort grown on calcareous grassland demonstrated higher allelopathic potential than extracts from underground parts and meadow habitat.

Our study aimed at active conservation of the last location of <em>Primula farinosa</em>, an endangered species in Poland, and assessed reproduction by seeds and plant propagation on sterile media in tissue culture conditions. We identified gibberellic acid (GA<sub data-id="subscript-1">3</sub>) as the key factor stimulating germination of <em>P. farinosa</em> seeds. Growing juvenile plants under controlled temperature of 18/16 °C day/night yielded good quality plant material without mycorrhization. In tissue culture, the most favorable medium for shoot propagation was MS supplemented with the lowest tested concentration of indole-3-butyric acid (IBA; 0.05 mg dm<sup data-id="superscript-1">−3</sup>) and 6-benzyl-aminopurine (BAP; 0.1 mg dm<sup data-id="superscript-2">−3</sup>). The rooting ability of shoots was high and comparable for all auxins used. 2C DNA content of seed-derived and micropropagated plants did not indicate any change in the ploidy level during in vitro cultivation. Plants derived from seeds and tissue cultures were compared in a 2-year study. Of all the characteristics compared, only the number of flowers per inflorescence was lower for micropropagated plants when compared with the seed-origin plants in the first year of observation. The difference was of transient nature and was not observed in the second year of the study. Effective protocols for in vivo and in vitro propagation of <em>P. farinosa</em> were developed, which can be used in practical species protection.