Method ISTA SDS-PAGE was used for separation, detection and evaluation of high molecular weight glutenin subunits (HMW) in the different wheat species. The relation has been studied between the HMW glutenin subunit alleles and the bread-making quality of 25 world wheat cultivars and 21 regional varieties common wheat varieties (Triticum aestivum L.), 17 winter spelt wheat (Triticum spelta L.), 3 durum wheat cultivars (Triticum durum DESF.), 9 cultivars of Triticum turgidum L. and 5 cultivars of Triticum polonicum L. The highest frequency of occurrence of HMW glutenin subunits 2*, 13 + 16 and 5 + 10 were found in world wheat cultivars. In Slovak wheat varieties were analysed subunits 0, 7 + 9 and 5 + 10, 2 + 12. The HMW subunits 0, 7 + 8 with Glu-score 4 were determined in Triticum durum DESF. Three electrophoretical profile groups of different HMW glutenin subunits were found in Triticum turgidum L. and Triticum polonicum L. and six electrophoretical profile groups were determined in Triticum spelta L. The verified correlations between bread-making quality and specific HMW subunits of glutenin can be utilised by wheat breeders using SDS-PAGE of proteins as a screening test for the prediction of bread-making quality of wheat. The glutenin proteins of wheat, particularly the highly aggregative glutenin fraction (HMW) strongly influence the bread-making properties of flours. The HMW glutenin subunits are encoded by genes at three genetically unlinked loci Glu-A1, Glu-B1, and Glu-D1, located close to the centromere on the long arm of the homoeologous chromosomes 1A, 1B, and 1D, respectively. There is the significant relationship between the presence and the absence of some specific HMW glutenin subunits and the bread-making quality of wheat varieties (Payne and Lawrence 1983, Payne et al. 1987, Vlasák 1995, aek and Èerný 1997. The most important subunits are 5 + 10 and 2 + 12, which are expressed from Glu-D1 loci. Method of SDS-PAGE is possible to apply for separation, detection and evaluation of high molecular weight glutenin subunits in wheat. KeywordsThe aim of our present contribution was to determine the type of the HMW glutenin subunits and to calculate the Glu-score in the grain of different species of wheat Triticum aestivum L., Triticum spelta L., Triticum durum DESF., Triticum turgidum L. and Triticum polonicum L. MATERIAL AND METHODSThe relationship between the HMW glutenin subunit alleles and the bread-making quality of different wheat species has been studied. The winter wheats included 25 world wheat cultivars and 21 regional varieties common wheat varieties (Triticum aestivum L.), 17 winter spelt wheat (Triticum spelta L.), 3 durum wheat cultivars (Triticum durum DESF.), 9 cultivars of Triticum turgidum L. and 5 cultivars of Triticum polonicum L. which were obtained from the Slovak Republic Genbank and from the Czech Republic Genbank. The cultivars Neepawa, Chinese spring, and Marquist were used as standards.For identification and characterisation of wheat varieties the standard vertical disc...