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We performed experimental and clinical MR studies to determine the optimal imaging conditions for the uterine three-layer structure on fast spin-echo T2-weighted imaging. In the experimental study, we used our original phantom. Imaging studies were carried out with different TRs, TEs, and ETLs, and the contrast-to-noise ratio (CNR) was evaluated. TR was the most important factor for contrast in both the phantom and clinical studies. The longer TR was, the better the contrast became. In the clinical study, TRs of 5500-6000 msec provided clear visualization of the uterine three-layer structure. Longer TRs would be useful for the detection of disease, differential diagnosis, and grading in patients with uterine diseases.