L-ascorbyl-2-monophosphate-Na/Ca (AMP-Na/Ca) was used as a vitamin C source to investigate its ascorbic acid (L-AA) enrichment and retention in boosted Artemia biomass (AB) and squid mantle muscle (SM). Different doses of AMP-Na/Ca (500, 1000, and 1500 AMP-Na/Ca mg/kg) were gradually dissolved into the culture tanks at time 0 (T 0 ) and at each hour until Hour 6 (T 6 ). Samples of AB and SM were taken for AMP-Na/Ca and L-AA analysis at T 0 , T 1 , T 2 , T 3 , T 4, T 5 , T 6 , T 12 , and T 24 . There were no significant differences (P > 0.05) among the AB groups at T 1 . The T 6 enrichment analysis for AB resulted in significant differences (P < 0.05) in the AMP-Na/Ca content for the 1500 mg/kg treatment, in which the initial concentration (0.001 ± 0.002 mg/kg) increased by more than 16-fold. For all AB enrichment treatments, the AMP-Na/Ca content demonstrated a decrease (32-11%) for the T 6 , T 12 and T 24 analysis. The T 1 analysis for SM at the higher AMP-Na/Ca enrichment concentration registered 30 mg/kg of L-AA and decreased (27.6%) at T 6 . This study demonstrated that AB and SM can be boosted with AMP-Na/Ca.