Antibiotic resistance is a global problem of modern medicine. In the research a microflora of out-patient department visitors in 2018–2019 was examined. It was revealed that the most often microbes were Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli. The dynamics of bacterial resistance to antibiotics was also analyzed. The multiple bacterial resistance to antibiotics were detected. The data show a rapid resistance genes spread among non-hospital strains, which necessitates constant monitoring of antibiotic sensitivity in order to develop the right tactics to combat antibiotic resistance.