A considerable amount of squid mantle collagen, 45% to 70% of total collagen, was not solubilized even after 30 min of heating in boiling water with its fibrous structure left intact. Pyridinoline, one of the major intermolecular crosslinks in matured collagen, was more predominantly included in the insoluble collagen than in the soluble one (p , 0.05). These results suggest that pyridinoline is closely related to the heat solubility of squid collagen.

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