This study provides a scientific information about the aqueous and methanol extracts of Rubia tinctorum based on its antimicrobial potential against gram positive and gram negative bacteria isolated from burns infection using the broth dilution and well diffusion method. Results of this study indicate the presence of many phytochemicals which have antimicrobial activity against broad spectrum of bacteria. The methanol extract of R.tinctorum showed highest activity than aqueous ones. The minimum inhibitory concentration (MIC) of the aqueous extract on the tested organisms was 25-100mg/ml while in the methanol extract ranged between 25-50mg/ml on the tested organisms and the minimum bacterial concentration (MBC) of the aqueous extract was 25-200 mg/ml while the methanol extract ranged between 25-100 mg/ml. The highest activity of methanol extract demonstrated at 100 C o , 121 C o agai nst S.aureus, K.spp, A.hydrophila, and S.marcescens, while there was low activity against S.dysenteria and E.coli. The activity of plant extract increased at acidic pH5-3 whearus, there are slightly increased of plant extract at alkaline pH 8. R.tinctorum contained. essential element (Pb, Na, K, Ca, Fe, Zn, P, Mn, Co and Cu) at different concertation. The high preformance liquid chromatograph (HPLC) analysis of R. tinctorum showed some chemical compounds that have antimicrobial activity against test isolates. The result of this study demonstrate that HPLC analysis of R.tinctorum constituent revealed that htis plant have antimicrobial activity against test organism and this may be suggest the use at this extract in treatment of infections disease.

No abstract

Ipomoea cairica cell cultures produced a tetrahydrofuran lignan, (þ)-pinoresinol, identified by UV, IR, MS and NMR methods, not yet found in the intact plant, and new in the Convolvulaceae family. Pinoresinol was found to have antioxidant and Ca 2þ antagonist properties. As it could be requested for its biological activity, we examined the possibility to raise the pinoresinol yield of I. cairica cultures, as well as we continued investigations on lignans' response to optimization.

No abstract

Our objectives were to establish a GC method capable of quantitative analysis of terpenoids without derivatisation and to examine the amount of β-sitosterol extracted from Morus alba L. leaf and stem bark by use of traditional organic solvent extraction and supercritical-fluid extraction (SFE). To measure β-sitosterol content without derivatization, GC-FID was used with 5-α-cholestan-3-one as internal standard. To identify terpenoid constituents, GC-MS was used; β-sitosterol, phytol, lanost-7-en-3-on, α-amyrin, βamyrin, and lupeol were identified. We established that for Morus leaf the best SFE method for β-sitosterol was pilot scale SFE; the β-sitosterol content of this extract was higher than that of the hexane solvent extract. Among analytical SFE conditions, 200 bar for 90 min and 300 bar for 60 min resulted in extraction of the most β-sitosterol. For mulberry stem bark, solvent extraction with hexane and SFE at 400 bar and 40°C for 60 min proved the best methods.