This study shows significant changes in the irrigation protocol applied in Serbian dental community. After 3 years of observation, NaOCl became widely accepted as the irrigant of choice, whereas H2O2 lost its popularity.

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Background/Aim. The studies of hydroxyapatite (HAp) and growth factors as the materials used for direct pulp capping have produced conflicting results for both the issue of inflammmatory response and the issue of calcified bridge formation. Calcium hydroxyapatite/poly(lactide-co-glycolide) is a bioresorbable polymer with demonstrated good characteristics as the carrier for bone morphogenetic protein (BMP) necessary in bone tissue regeneration. The role of growth factors in dental tissue reparation (in both reactionary and reparative dentinogenesis) represents the new foundation and provides a different approach to dental pulp treatment. Growth factors-TGF-beta 1directly induce morphological and functional differentiation of neodontoblasts. The aim of this experimental study was to investigate the effect of calcium hydroxyapatite/poly(lactide-coglycolide) HAp/PLGA and growth factors (TGF-β1) in the formation of a calcified tissuedentine bridgeon the teeth of our experimental model. Methods. Rodent (rabbit) teeth were used as the animal model. After the trepanation of pulp space with sterile steel drills, the pulp was capped with calcium hydroxyapatite/poly(lactide-co-glycolide) HAp/PLGA (experimental group I; n=60); calcium hydroxyapatite/poly(lactide-co-glycolide) HAp/PLGA combined with TGF-β1 growth factor (experimental group II; n=60), and there was a control group of intact teeth (n=20). The experiment was performed in general anesthesia. The animals were kept alive for 1, 3 and 6 months. The extracted teeth were adequately prepared for scanning electron microscopy.Results. Scanning electron microscopy (SEM) demonstrated that the number of teeth with calcified tissue in the form of dental bridges in the HAp/PLGA+TGF-β1 group was statistically significantly greater 6 months (66.67%) than 3 months after the treatment (26.67%), at the statistical significance level of p<0.05. Conclusion. Direct pulp capping covers the artificially exposed dental pulp and makes possible the formation of a dentine bridge (a tubular structure composed of reparative dentine) in the period of 3 months.

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<Zakljucak> Dobro poznavanje mogucih uzoraka prekomernog krvarenja, kao i stomatoloskih postupaka u toku oralnohirurskih intervencija kod obolelih od trombocitopenije, hemofilije, u slucaju snizene koncentracije faktora zavisnih od vitamina K i predozirane antitrombotske terapije, omogucava adekvatnu i blagovremenu oralnohirursku hemostazu u skladu sa predvidjenim protokolom lecenja. Ukoliko je, medjutim, oralnohirurska intervencija propracena produzenim krvarenjem u trajanju od nekoliko sati ili dana, bez pouzdanih anamnestickih podataka i pismene medicinske dokumentacije, problem postaje veliki. U takvim situacijama, podsecanje na stanja koja izazivaju prekomerno krvarenje, kao i na stomatoloske postupke u toku oralnohirurskih intervencija kod takvih bolesnika, moze biti dragocen doprinos dobroj stomatoloskoj praksi.