Northern India is one of the oldest geophysical regions of human evolution and migration in the world. Genetic and anthropological studies have shown that the peopling the subcontinent is characterized by a complex history, contributed from different ancestral populations. Genetic level studies of polymorphisms always associated with health and diseases of population becoming the need of hours. The present study was aimed to explore the relationship of ABO blood groups association of populations and to assess the prevalence of blood groups in different categories of Northern India and to compare our results with other studies conducted in India. Blood samples from 155 unrelated individuals was collected from Naga tribal settlements Blood groups of individuals were evaluated with the presence of antigen using monoclonal antibody by a standard blood typing are protocol, Gene frequencies was calculated by Hardy-Weinberg method. The blood group O 67 (43.23%) was the commonest group prevalent in donors followed by group B 45 (29.03%), A 33(21.29%) and AB 10 (6.45%). The Rh negative was not observed in female donors, whereas in male it was found to be 1.3%.Data among tribal suggest their common origin as well as a drift from an original population due to the possible founder effect among tribal Naga Ten Tribal