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The effects of estradiol dipropionate (EDP) or calcium glucoheptonate (Ca) on the morphology and stereology of the PRL cells in 14-month-oldWistar female rats were studied. The animals were treated daily with EDP in the dose of 0.625 mg/kg b.w. or calcium glucoheptonate (Ca; 11.4 mg/kg b.w.) for two weeks. The controls were injected with vehicle alone by the same schedule. Mammotrophs (PRL cells) were immunocytochemically localized by the PAP method. Blood PRL concentration was determined by Delfia procedure. In animals treated with EDP the volume of both, PRL cells and their nuclei, as well as the volume densities were significantly (p<0.05) increased by 17%, 9% and 38%, respectively, in comparison with the controls. In animals treated with Ca all morphometric parameters were insignificantly (p>0.05) decreased compared to control rats. Serum concentration of PRL was significantly increased (p<0.05) by 17% after estradiol treatment, but in Ca-treated females this parameter was insignificantly (p>0.05) changed by 2% compared to controls. Based on these results, it can be concluded that EDP expresses a strong stimulatory effect on the morphology and function of pituitary PRL cells

Introduction. Direct pulp capping procedure is a therapeutic application of a drug on exposed tooth pulp in order to ensure the closure of the pulp chamber and to allow the healing process to take place. Objective. The aim of this study was to examine the histological effects of Emdogain? on exposed tooth pulp of a Vietnamese pig (Sus scrofa verus). Methods. The study comprised 20 teeth of a Vietnamese pig. After class V preparation on the buccal surfaces of incisors, canines and first premolars, pulp was exposed. In the experimental group, the perforations were capped with Emdogain? (Straumann, Basel, Switzerland), while in the control group pulp capping was performed with MTA? (Dentsply Tulsa Dental, Johnson City, TN, USA). All cavities were restored with glass-ionomer cement (GC Fuji VIII, GC Corporation, Tokyo, Japan). The observational period was 28 days, after which the animal was sacrificed and histological preparations were made. A light microscope was used to analyze dentin bridge formation, tissue reorganization and inflammation, and the presence of bacteria in the pulp. Results. The formation of dentin bridge was observed in the experimental and control groups. Inflammation of the pulp was mild to moderate in both groups. Angiogenesis and many odontoblast-like cells, responsible for dentin bridge formation, were observed. Necrosis was not observed in any case, nor were bacteria present in the pulp. Conclusion. Histological analysis indicated a favorable therapeutic effect of Emdogain? Gel in direct pulp capping of Vietnamese pigs. Pulp reaction was similar to that of MTA?. [Projekat Ministarstva nauke Republike Srbije, br. ON172026]