A novel fluorescent turn-on chemosensor DHADC ((E)-3-((4-(diethylamino)-2hydroxybenzylidene)amino)-2,3-dihydrothiophene-2-carboxamide) has been developed and used to detect Zn 2+ and CN − . Compound DHADC displayed a notable fluorescence increase with Zn 2+ . The limit of detection (2.55 ± 0.05 µM) for zinc ion was far below the standard (76 µM) of the WHO (World Health Organization). In particular, compound DHADC could be applied to determine Zn 2+ in real samples, and to image Zn 2+ in both HeLa cells and zebrafish. Additionally, DHADC could detect CN − through a fluorescence enhancement with little inhibition with the existence of other types of anions. The detection processes of compound DHADC for Zn 2+ and CN − were demonstrated with various analytical methods like Job plots, 1 H NMR titrations, and ESI-Mass analyses.

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