In high-density aquaculture, fish health can suffer because of excessive feeding, which causes fatty liver disease. Siberian ginseng (Acanthopanax senticosus) has been used as a feed additive to promote animal growth, immunity, and lipid metabolism. In this study, we explored the effects of A. senticosus on the physiology of hybrid yellow catfish (Tachysurus fulvidraco ♀ × Pseudobagrus vachellii ♂). A control group and five groups fed diets containing A. senticosus (0.5, 1, 2, 4, and 8 g A. senticosus/kg feed) were established and maintained for 8 weeks. Dietary supplementation with A. senticosus at 4 g/kg promoted growth of the hybrid yellow catfish. Serum total cholesterol (TC) and triacylglycerol (TG) levels at 2 g/kg A. senticosus (TC: 1.31 mmol/L; TG: 1.08 mmol/L) were significantly lower than in the control group (TC: 1.51 mmol/L; TG: 1.41 mmol/L), and 4 g/kg A. senticosus (17.20 μmol/g tissue) reduced the liver TG level compared with the control group (21.36 μmol/g tissue) (P <0.05). Comparative transcriptomic analysis of liver tissue between the control group and the group showing optimum growth (4 g/kg A. senticosus) revealed 820 differentially expressed genes and 44 significantly enriched pathways, especially lipid metabolism pathways such as unsaturated fatty acid and fatty acid metabolism. The transcript levels of five lipid metabolism-related genes were determined by quantitative real-time PCR. The results showed that 2–4 g/kg A. senticosus supplementation reduced the FADS2, ELOVL2, CYP24a, and PLPP3 transcript levels and 4 g/kg A. senticosus increased the DIO2 transcript level (P <0.05), leading to altered synthesis of TG and thyroxine and reduced fat deposition in the liver. Our results show that dietary A. senticosus affects the regulation of fat metabolism and promotes the growth of hybrid yellow catfish. A. senticosus is a healthy feed additive, and the appropriate dietary supplementation rate is 2–4 g/kg.
Mechanical Biological Treatment Process of MSW is a new garbage disposal technology for the main purpose to improve the efficiency of garbage sorting. Malodorous gas, namely provisions to limit emissions of the emitted pollutant discharge standard was collected and analyzed, and 64 major VOCs emissions in the process of MBT were also analyzed by gas chromatography-mass spectrometry (GC-MS). The results showed that, First, odor concentration of the top ten odorous substances of VOCs in MSW were successively toluene, ethyl acetate, methylene chloride, methyl ethyl ketone, xylene, styrene, 1,2- dichloropropane, trichlorethylene, ethylbenzene, trimethylbenzene, Accounted for 93.57% of the volume of total VOCs. Therefore, the above VOCs were key control object during MBT process; Second, at trommel link of Mechanical separation process, ammonia, hydrogen sulfide, carbon disulfide, dimethyl sulfide odor concentration were higher 11.67%, 3.8%, 25.42%, 128.93% compared to artificial selection as background value; Third, during biological treatment processes, NH3, H2S were the main control substances. ketones was detected in the end of the biological treatment, indicating that local anaerobic reactor engendered in the composting process. Therefore, proper ventilating was needed to control VOCs production and release in biological process.
In this paper the effects of Ca2+ and Mg2+ on the floatability of smithsonite and hemimorphite were investigated through microflotation. The flotation tests were performed using purified samples from Lanping mine of China by the microflotation technique. The Ca2+ flotation results showed that the recovery of smithsonite decreased from 89.81% to 83.92% and the recovery of hemimorphite decreased from 84.83% to 76.03% when increasing Ca2+ concentration from 200mg/L to 1000mg/L. Also, while increasing Mg2+ concentration from 200mg/L to 1000mg/L the smithsonite and hemimorphite recoveries declined to reach 80.03% and 71.75%, respectively. It can be concluded that the recoveries of smithsonite and hemimorphite showed a decreasing trend with the increasing of Ca2+ and Mg2+ concentration, and the effect on the recovery of hemimorphite was larger. Moreover, the effects of Mg2+ on the smithsonite and hemimorphite are greater in the same test condition, which could be attributed to the solubility product difference of Ca (OH)2and Mg (OH)2.
Overnutrition in high-density aquaculture can negatively affect the health of farmed fish. The Chinese herbal medicine Siberian ginseng (Acanthopanax senticosus, AS) can promote animal growth and immunity, and regulate lipid metabolism. Therefore, we conducted an 8-week experiment, in which Oreochromis niloticus was fed with a diet supplemented with different concentrations of AS water extract (ASW) (0‰, 0.1‰, 0.2‰, 0.4‰, 0.8‰, and 1.6‰). The ASW improved the growth performance and increased the specific growth rate (SGR). Linear regression analysis based on the SGR estimated that the optimal ASW amount was 0.74‰. Dietary supplementation with 0.4–0.8‰ ASW reduced the triglyceride and total cholesterol levels in the serum and liver, and regulated lipid transport by increasing the high-density lipoprotein cholesterol concentration and lowering the low-density lipoprotein cholesterol concentration. Dietary supplementation with ASW increased the activities of superoxide dismutase and catalase in the liver, thereby improving the antioxidant capacity. Moreover, ASW modulated the transcription of genes in the peroxisome proliferator-activated receptor signaling pathway in the liver (upregulation of PPARα, APOA1b, and FABP10a and downregulation of PPARγ), thereby regulating fatty acid synthesis and metabolism and slowing fat deposition. These results showed that 0.4–0.8‰ ASW can slow fat deposition and protected the liver from cell damage and abnormal lipid metabolism.
The zinc oxide sample from the Lanping mine located in the west of Yunnan province,China, is characterized with low grade, high calcium content, high oxidation rate and heavy slime. According to the properties of the ore, a new dressing-metallurgy combined method is proposed to process the ore and the beneficiation part is discussed by this paper. Based on the regulation of reagent scheme, flotation flowsheet of pre-desliming and two rougher is conducted to obtain a zinc concentrate that Zn grade is 18.72% at Zn recovery of 90.88%. The calcium removal rate of zinc concentrate reaches to as high as 78.15% for the flotation operation, which could lay the foundation for the next acid leaching process.
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