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Egg substrates, including eggshell, egg membranes, and egg white, exert a significant influence on CaCO 3 biomineralization. CaCO 3 crystallization at different temperatures, concentrations, and with various egg substrates was systematically investigated via rapidly mixing solution method. The crystals were determined by scanning electron microscopy, Fourier transform infrared spectroscopy, and X-ray diffraction. As the CaCl 2 concentration increased, the CaCO 3 crystal form gradually changed from calcite to vaterite. Under rising temperature, the single-hole vaterite and besom-like aragonite were formed on the raw and boiled egg white substrate, respectively. The effects of electric field on CaCO 3 crystallization were also explored. The findings may offer a novel approach to CaCO 3 synthesis.