LC-ESI-MS and HPLC were used for the identification of the constituents from G. lutea leaves collected at different localities, as well as for quantification of the main compounds. Seven secoiridoids, five C-glucoflavones and three xanthones, were identified. Swertiamarin derivatives, namely eustomorusside (2), eustomoside (3) and septemfidoside (5), were detected in G. lutea for the first time. Concentrations of five constituents (swertiamarin, gentiopicrin, isovitexin, mangiferin and isogentisin) were determined. The relationship between concentrations of γ-pyrones and altitude was observed with statistically significant correlation (r = 0.94). The extracts were also evaluated for their content of total phenolics, and antiradical and cytotoxic activities. The total phenolics content ranged from 7.7 to 12.7 mg GAE/g, and the IC 50 values for DPPH radical scavenging activity varied between 0.45 to 2.02 mg/mL. The leaf extract exhibited moderate cytotoxic effects toward HeLa cells with an IC 50 value of 41.1 µg/mL, while gentiopicrin, mangiferin and isogentisin exerted strong activity against HeLa cells, with IC 50 values ranging from 5.7 to 8.8 µg/mL. The results confirm the traditional usage of G. lutea leaves and also suggest their possible utilization as hepatoprotective, hypoglycemic and anti-inflammatory agents.

This paper presents the five-year long research results (2007-2011) of phenological observations (beginning, full flowering and the end of flowering), morphometric analysis (fruit weight, length, width and thickness of fruit, as well as length and width of the hilum), the ripening time and the average yield of selected genotypes of sweet chestnut (Castanea sativa Mill.). The results show that in terms of time of flowering chestnut trees examined in the group are very early flowering. The ripening of studied chestnut trees shows that the earliest harvest tree was Ostros II, VII and Kostajnica V (09.11.), and Kostajnica III (14.11.) were the latest. The average fruit weight of the selected sweet chestnut trees was (6.9 g). The highest average fruit weight (10.6 g), had the examined Ostros I tree, and the lowest (4.8 g), Kostajnica VI. The yield of the tested chestnut trees was on average (76.3 kg / tree). The selected trees Kostajnica II (66.0 kg / tree), Kostajnica I (69.0 kg / tree) had the smallest yield and Ostros VI (94.0 kg / tree) and Ostros V (87.0 kg / tree) had the highest. Obviously it can be concluded that population genetic variability is very high and gene expression is highly affected in tested samples on both locations. For further research it should be given attention to investigate genotypes in controlled conditions, the best in vitro in tissue culture. [Projekat Ministarstva nauke Republike Srbije, br. TR-20013 i br. TR-31064

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Collecting medicinal plants from their natural habitats cannot meet the needs of the increased demand for natural medicinal raw materials in the global market, which caused the expansion of medicinal plants cultivation. This paper deals with the influence of the three varieties of pot marigold; "Plamen Plus", "Bački Petrovac" and "Orange King" in different sowing density on pot marigold yield.By studying the effects of the variety and plant density on a total yield of fresh inflorescence of marigold, it was found out that variety has a statistically significant effect on yield. The variety "Plamen plus" has achieved the higher yield (5724 kg ha -1 ) comparing with the other two varieties which did not differ significantly, although the lowest yield was obtained by the variety "Orange King" (3243 kg ha -1 ).

SažetakU radu su prikazani rezultati za prinos zrna sorti heljde gajene na području dva lokaliteta (Pašića Polje i Laholo) u opštini Bijelo Polje, Crna Gora. Istraživanje je izvedeno u mikroogledima, koji su urađeni po slučajnom blok sistemu u četiri ponavljanja tokom 2010. godine. Heljda je gajena po principima ekološke proizvodnje. U ogledu je bilo zastupljeno 11 sorti (Novosadska, Heljda 2, Bamby, Češka, Darja, Prekmurska, Čebelica, Francuska, Heljda1, Spacinska i Godijevo). Kao standardna sorta korišćen je tip Godijevo. Ustanovljene su statistički značajne razlike u prinosu izmedju ispitivanih sorti i lokaliteta. Na lokalitetu "Pašića Polje" prosječan prinos zrna iznosio je 511,86 kg ili ha -1 za 54,14 kg manje od standarda-tipa Godijevo. Na ovom oglednom polju najviši prinos ostvarila je sorta Heljda 2 (619,25 kg ha ֿ◌¹), sa 53,25 kg više u odnosu na standardnu sortu Godijevo (566,00 kg ha -1 ). Najniži prinos ostvarila je sorta Heljda 1 (308,75 kg ha -1 ) sa 257,25 kg manje od standardne sorte. Na ovom oglednom polju ("Pašića Polje") ostvareni su znatno niži prinosi zrna u poređenju sa oglednim poljem "Laholo". Na lokalitetu "Laholo" prosječan prinos zrna izosio je 784,70 kg ha -1 ili za 134,30 kg manje od standarda-tipa Godijevo. Na ovom oglednom polju najviši prinos ostvarila je sorta Francuska (964,75 kg ha -1 ) sa 45,75 kg više u odnosu na standardnu sortu Godijevo (919,00 kg ha -1 ). Najniži prinos ostvarila je sorta Heljda 1 (455,25 kg ha -1 ), što je čak za 463,75 kg manje od standardne sorte. Na ovom oglednom polju ("Laholo") ostvareni su znatno veći prinosi u poređenju sa oglednim poljem "Pašića Polje". Ustanovljena je značajna razlika u prinosu zrna izmedju ispitivanih lokaliteta. Najveća razlika utvrđena je kod sorti Francuska, Prekmurska i Godijevo, a najmanja kod sorte Heljda 1.