ORFΔcoq2Δ, yeast double mutant harboring a gene deletion in a designated open reading frame plus a deletion in COQ2; pABA, para-aminobenzoic acid; PC, phosphatidylcholine; Pyr12, 1-pyrene dodecanoic acid; SD, synthetic dextrose medium; vCLAMP, vacuole-mitochondria patch; WT, wild-type parental yeast strain; YPD, rich growth medium containing dextrose as a fermentable carbon source; YPG, rich growth medium contain glycerol as the sole non-fermentable carbon source.