Objective: This research was study about phytochemical active phenolic compound from pods of jiringa (Archidendron jiringa (Jack) I. C. Nielsen) and it's antioxidant. Methods:Pods of jiringa were air dried and macerated with methanol. Extract was evaporated using rotary evaporator, and then, crude extract dissolved with water and partitioned with ethyl acetate. Extracts were evaporated and partitioned again with methanol and n-hexane. Column and preparative chromatography used to separate pure compound. Pure compound as methyl gallate was identificated with data from nuclear magnetic resonances of proton H ( 1 H NMR), NMR of carbon ( 13 C NMR), and mass spectrometry (MS). Antioxidant activity of pure compound tested by 1,1-diphenyl-2-picrylhydrazyl method. Results:We found pure phenolic compound from pods of jiringa as methyl gallate that exists on fraction III-2, and it has high antioxidant activity with inhibition concentration 50 was 3.7576 µg/ml. Conclusions:Pods of jiringa contain of active phenolic compound as methyl gallate that has high antioxidant activity. Therefore, it can be used as a source of natural antioxidant.
Objective: This research was study about phytochemical active phenolic compound from pods of jiringa (Archidendron jiringa (Jack) I. C. Nielsen) and it's antioxidant. Methods:Pods of jiringa were air dried and macerated with methanol. Extract was evaporated using rotary evaporator, and then, crude extract dissolved with water and partitioned with ethyl acetate. Extracts were evaporated and partitioned again with methanol and n-hexane. Column and preparative chromatography used to separate pure compound. Pure compound as methyl gallate was identificated with data from nuclear magnetic resonances of proton H ( 1 H NMR), NMR of carbon ( 13 C NMR), and mass spectrometry (MS). Antioxidant activity of pure compound tested by 1,1-diphenyl-2-picrylhydrazyl method. Results:We found pure phenolic compound from pods of jiringa as methyl gallate that exists on fraction III-2, and it has high antioxidant activity with inhibition concentration 50 was 3.7576 µg/ml. Conclusions:Pods of jiringa contain of active phenolic compound as methyl gallate that has high antioxidant activity. Therefore, it can be used as a source of natural antioxidant.
Objective: This study was aimed to isolate and investigate antioxidant activity of gallic acid in pods of jiringa (Archidendron jiringa [Jack] I.C. Nielsen).Methods: Pods of jiringa were extracted by maceration. Phenolic compounds were tested using FeCl3. Identification of pure compound was obtained from spectra data from nuclear magnetic resonance of proton H (1H NMR), NMR of carbon (13C NMR), infra-red, and mass spectrometry. Antioxidant activity was investigated using 1,1-diphenyl-2-picrylhydrazyl method.Results: The IC50 of gallic acid from jiringa’s pods (A. jiringa [Jack] I.C. Nielsen) was 3.65 μg/ml. This value showed that gallic acid from jiringa’s pods (A. jiringa [Jack] I.C. Nielsen) had high antioxidant activity.Conclusions: Gallic acid presents in pods of jiringa (A. jiringa [Jack] I.C. Nielsen) and has high antioxidant activity.
Pemanfaatan kulit biji jengkol masih sangat terbatas, bahkan sering ditemukan menjadi tumpukan sampah organik di pasar-pasar tradisional daerah Sumatera Utara. Penelitian ini bertujuan untuk menyelidiki kandungan senyawa fenolik yang terdapat pada kulit biji jengkol. Senyawa fenolik memiliki aktivitas antioksidan yang dapat mencegah beberapa jenis penyakit. Pada penelitian ini diperoleh ekstrak metanol, ekstrak etil asetat, ekstrak n-heksan dan total fenolik dari kulit biji jengkol dan dilakukan uji toksisitas menggunakan metode Brine Shrimp Lethality Test (BSLT). Nilai LC50 yang diperoleh untuk ekstrak metanol, ekstrak etil asetat, ekstrak n-heksan dan total fenolik secara berturut-turut adalah 14,91; 14,19; 3419,64 dan 10,44 ppm. Nilai LC50 dipengaruhi oleh kandungan senyawa fenolik yang ada pada ekstrak. Nilai LC50 ekstrak metanol, ekstrak etil asetat dan fenolik total menunjukkan nilai toksisitas yang tinggi. Berdasarkan pada nilai LC50 yang diperoleh, disimpulkan bahwa kulit biji jengkol dapat digunakan sebagai sumber obat herbal untuk pengobatan berbagai jenis penyakit.
Penelitian ini dilakukan untuk menentukan aktivitas antioksidan fenolik total dari ekstrak kulit biji jengkol. Kulit biji jengkol dalam bentuk serbuk yang sudah dikering angin anginkan selama 1x24 jam dimaserasi dengan metanol selama 1x24 jam. Ekstrak yang diperoleh dilarutkan dengan air secara berulang-ulang dan kemudian fraksi air dipartisi dengan etil asetat berulang-ulang. Selanjutnya ekstrak pekat dilarutkan dengan metanol dan diparitsi dengan n-heksan untuk memperoleh fenolik total. Metode DPPH digunakan untuk menentukan aktivitas antioksidan. Nilai IC 50 yang diperoleh adalah 11,7987. Nilai ini menunjukkan aktivitas antioksidan yang cukup tinggi. This study was conducted to determine the total phenolic antioxidant activity of jengkol seed skin extract. Jengkol seed slayer in the form of powder that had been dried for 1 x 24 hours macerated with methanol for 1 x 24 hours. The extract was dissolved with water repeatedly and then the fraction of water was partitioned with ethyl acetate repeatedly. Then the concentrated extract was dissolved with methanol and paritized with n-hexane to obtain the total phenolic. The DPPH method was used to determine antioxidant activity. The IC 50 value obtained was 11.7987. This value showed quite high antioxidant activity.
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