Urinary tract infections(UTI) are the most common problem caused by infections in the urinary system, which includes kidneys, bladder, ureters and urethra. Urinary infections are found to be one of the common diseases worldwide. The study mainly focuses on analyzing the association of TPA 25 gene with Urinary tract infected individual in tribal population of Dimapur District. This study considers the population of Urinary tract infection(UTI)patients. The samples of the study consist of 32 Urinary tract infected patient which was collected from Eden hospital, Dimapur and 32 control samples through random sampling technique. The genotyping is done using Polymerase Chain Reaction (PCR) with allele specific marker (TPA 25). The study concluded that the association of TPA gene is significantly high in both ID and DD genotype (0.00*) of the male population and females shows statistical significant in ID and II phenotype (0.002*).