No abstract

A simple spectrophotometric method was used to determine the phosphate content in the water sampels. The method is based on the formation of molybdenum blue complex from the reaction of orthophosphate and ammonium molybdate followed by reduction with ascorbic acid in the aqueous sulfuric acid medium. The color intensity of the molybdenum blue complex is proportionally to the phosphate content in the solution. The system obeys Lamber-Beer’s Law at the 890 nm in the concentration range of 0.1-1 mg P/L and the linier calibration graph was obtained with the slope, 0.6334, intercept, 0.0074 and correlation coefficient of 0.9988. This official method was also used for the quantitative analysis of phosphate in the water samples and the satisfactory result was obtained. The range of phosphate concentration in the water samples are 0.033 – 2.943 mg P/L.

A simple spectrophotometric method was used to determine the phosphate content in the water sampels. The method is based on the formation of molybdenum blue complex from the reaction of orthophosphate and ammonium molybdate followed by reduction with ascorbic acid in the aqueous sulfuric acid medium. The color intensity of the molybdenum blue complex is proportionally to the phosphate content in the solution. The system obeys Lamber-Beer’s Law at the 890 nm in the concentration range of 0.1-1 mg P/L and the linier calibration graph was obtained with the slope, 0.6334, intercept, 0.0074 and correlation coefficient of 0.9988. This official method was also used for the quantitative analysis of phosphate in the water samples and the satisfactory result was obtained. The range of phosphate concentration in the water samples are 0.033 – 2.943 mg P/L.

Personal protective equipment (PPE) is a device that has the ability to protect a person whose function is to isolate part or all of the body from potential hazards in the workplace. Knowledge and attitudes have a considerable influence on the use of PPE. If workers have good knowledge, workers will use PPE properly. The purpose of this study was to determine the relationship between the level of knowledge of occupational safety and health (K3) with the attitude of using personal protective equipment in the incense workers of PT. Kaori Group in 2021. This type of research is an analytical survey using the interview method with theapproach Cross Sectional, the sampling method used is the sampling technique.saturated. The sample amounted to 62 respondents. From the results of the Chi Square test, the value of p = 0,000 is obtained, because p = 0,000 0.05, it can be concluded that Ho is rejected and Ha is accepted. This means that there is a significant relationship between the level of knowledge about occupational safety and health with the attitude of using personal protective equipment for workers.ABSTRAKAlat Pelindung Diri (APD) adalah suatu alat yang memiliki kemampuan untuk melindungi seseorang yang fungsinya untuk mengisolasi sebagian atau seluruh tubuh dari potensi bahaya di tempat kerja. Pengetahuan dan sikap memiliki pengaruh yang cukup besar terhadap penggunaan APD. Jika pekerja memiliki pengetahuan yang baik, pekerja akan menggunakan APD dengan baik. Tujuan penelitian ini adalah untuk mengetahui hubungan antara tingkat pengetahuan keselamatan dan kesehatan kerja (K3) dengan sikap penggunaan alat pelindung diri pada pekerja kemenyan PT. Kaori Group tahun 2021. Jenis penelitian ini adalah survey analitik dengan menggunakan metode wawancara dengan pendekatan Cross Sectional, metode pengambilan sampel yang digunakan adalah teknik sampling jenuh. Sampel berjumlah 62 responden. Dari hasil uji Chi Square diperoleh nilai p = 0,000, karena p = 0,000 0,05 maka dapat disimpulkan bahwa Ho ditolak dan Ha diterima. Artinya ada hubungan yang signifikan antara tingkat pengetahuan tentang keselamatan dan kesehatan kerja dengan sikap penggunaan alat pelindung diri pada pekerja.

Introduction: Infectious mouth diseases are caused by microorganisms such as      Staphylococcus aureus and Streptococcus mutans. Sentul fruit peel extract contains several phytochemical compounds, flavonoid compounds, saponins and tannins are aromatic hydroxyl groups that act as antibacterial so that they can be used as a treatment for infections of the mouth. This study aimed to determine the inhibitory ability of Sentul fruit peel extract (Sandoricum koetjape) against Streptococcus mutans and Staphylococcus Aureus bacteria which can cause oral infections. Method: Sentul fruit peel simplicia was extracted by maceration method with ethanol for 24 hours. The maceration method was chosen in this study because it is a method that is easy to do and uses simple tools, which is enough to soak the sample in a solvent. A filtering process followed this and the filtrate was then evaporated with a vacuum rotary evaporator at a temperature of 45oC, so that a thick extract was produced. The maceration process was repeated 2 times. After the extraction process, then proceed with liquid-liquid fractionation using distilled water. The thick extract was put into a separating funnel and distilled water was added. Then it was shaken and the aquadest fraction was taken, followed by evaporation with a vacuum rotary evaporator at 45oC, so that the aqua fraction of the ethanol extract was produced. Result: The results showed that the Sentul fruit peel extract was able to inhibit the growth of      S. mutans and S. aureus bacteria as seen from the formation of a clear zone. Based on the ANOVA test, the extract concentration treatment had a significant effect (p<0.005) on the diameter of the clear zone on S. mutans and S. aureus bacteria. Positive control (streptomycin) showed significant differences in Duncan's test, because it produced the greatest antibacterial activity against test bacteria compared to negative control and various extract concentrations. The diameter of the clear zone in the positive control against S. mutans and S. aureus bacteria were 17.63±0.28 mm and 17.62±1.04 mm, respectively. Sentul peel ethanol extract of aqua fraction at a concentration of 100% gave the highest inhibition zone compared to concentrations of 75%, 50%, and 25%, although it was still smaller than the diameter of the clear zone in the positive control. In bacteria S. mutans, the ethanol extract of Sentul peel aqua fraction at a concentration of 100% gave a clear zone diameter of 14.31±1.06 mm and in S. aureus bacteria it gave a clear zone diameter of 15.34±1.81 mm. Conclusion: Sentul fruit peel extract (Sandoricum koetjape) has the ability to inhibit Streptococcus mutans bacteria and Staphylococcus Aureus bacteria.