Medicinal plants have played an essential role in the treatment of various diseases. Thymus vulgaris, a medicinal plant, has been extensively used for biological and pharmaceutical potential. The current study was performed to check the biopotential of active biological compounds. The GC-MS analysis identified 31 compounds in methanolic crude extract, among which thymol, carvacrol, p-cymene, and eugenol are the main phytoconstituents present in T. vulgaris. The HPLC analysis quantified that flavonoids and phenolic acids are present in a good concentration in the active fraction of ethyl acetate and n-butanol. FTIR confirmed the presence of functional groups such as phenols, a carboxylic group, hydroxy group, alcohols, and a benzene ring. Among both fractions, ethyl acetate showed high antioxidant activity in the DPPH (84.1 0.88) and ABTS (87.1 0.89) assays, respectively. The anti-inflammatory activity of the fractions was done in vitro and in vivo by using a carrageenan-induced paw edema assay, while the hexane-based extract showed high anti-inflammatory activity (57.1 0.54) in a dose-response manner. Furthermore, the lead compound responsible for inhibition in the denaturation of proteins is thymol, which exhibits the highest binding affinity with COX1 (−6.4 KJ/mol) and COX2 (−6.3 KJ/mol) inflammatory proteins. The hepatotoxicity analysis showed that plant-based phytoconstituents are safe to use and have no toxicity, with no necrosis, fibrosis, and vacuolar degeneration, even at a high concentration of 800 mg/kg body weight. Furthermore, the in silico analysis of HPLC phytochemical compounds against gastric cancer genes showed that chlorogenic acid exhibited anticancer activity and showed good drug-designing characteristics. Thrombolysis and hemolysis are the major concerns of individuals suffering from gastric cancer. However, the T. vulgaris fractions showed thrombolysis from 17.6 to 5.4%; similarly, hemolysis ranged from 9.73 to 7.1% at a concentration of 12 mg/mL. The phytoconstituents present in T. vulgaris have the potential for multiple pharmacological applications. This should be further investigated to isolate bioactive compounds that can be used for the treatment of different ailments.

Plants are playing important role in the planet by providing food for humans and stability in the environment. Phytohormones are key regulators in various physiological processes and among the most important small signaling molecules affecting plant growth and yield production. These biochemical also initiate adaptive responses caused by external stimuli, such as biotic and abiotic stress. Generally, on the basis of physiology, plant hormones roughly fall into two classes. In class one, phytohormones fall which is responsible for plants growth-promoting activities, such as cell division, cell elongation, seed and fruit development, and pattern of differentiation. On the other hand, the second class of hormone play important role in plants’ response, such as biotic and abiotic stresses. Some other hormones, such as jasmonates, salicylic acid, brassinosteroids, and strigolactones, also play a key role in plants. Their biochemical signaling network and their crosstalk ability make plant hormones excellent candidates to optimize plant growth and/or mediate abiotic and biotic stresses in agriculture. In the end, the future trends of plant hormone analysis are exploring plant hormones and their applications. We believe the perspective may serve as guidance for the research of plant hormones in the analytical, environmental, and botanical fields.

Background/ Aim: Pregnancy induced hypertension (PIH) has been a leading cause of maternal mortality worldwide. It could be responsible for several complications like hemodilution, altered lipid metabolism and deficiencies of different minerals in body. Although the causes of Pregnancy induced hypertension (PIH) remains indistinct. The aim of our present study was to evaluate the changes in magnesium serum levels and lipid profile in hypertensive and normotensive pregnant women. Materials and Methods: An analytical case control study comprising 60 individuals was designed. From which 30 normotensive pregnant women served as control group (A) and 30 pregnancy induced hypertensive women served as case group (B). Patients with pre-existing hypertension were excluded. Results: The independent t-test was used for comparison of various parameters between group A and B. Triacylglycerol (TAG) and low density lipoprotein cholesterol (LDL-C) levels were significantly high in PIH (B) as compare to normotensive group (A). Mean serum values of TG were measured as (195.73 ± 70.28 vs 156.27 ± 55.60 mg dL-1) and LDL-C vs control (87.33 ± 37.74 vs 69.45 ± 28.58 mg dL-1) in PIH and normotensive groups respectively. HDL-C in PIH vs Control (47.40±1.12 vs 54.43 ±1.27 mg dL-1) and Mg+2 PIH vs control (1.36±0.07 vs 1.94±0.35 mg dL-1) were measured respectively. High density lipoprotein cholesterol (HDL-C) and magnesium Mg+2 levels were significantly reduced in PIH. Conclusion: The lower serum magnesium levels and abnormal lipids profile may indicate their possible role as risk factors in the development of PIH in pregnant women. Hence initial diagnosis of hypomagnesemia in PIH cases may help in minimizing the complications and development of new cure strategies.

Background: Dengue virus (DENV) is an RNA virus belonging to the family Flaviviridae of the genus Flavivirus with worldwide distribution. Dengue fever is caused by any of four closely-related serotypes DENV, an emerging pandemic-prone viral disease in many regions of the world. Objectives: The present study aimed to determine the prevalence of dengue virus genotypes and serotypes in children aged below 15 years in Lahore, Pakistan. Methods: In this study, 112 serum samples were collected from clinically suspected dengue fever patients from March 2017 to December 2018 at different tertiary care hospitals in Lahore, Pakistan. Regarding the patients’ age, the samples were divided into four groups from A to D (i.e., 0 - 1, 1 - 5, 5 - 10, and 10 - 15 years of age). Rapid immuno-chromatography (ICT) test was conducted on the collected serum samples, followed by quantitative RT-PCR for serotype of dengue virus. Results: Out of 112 samples, 34 samples were diagnosed as DENV positive by the rapid ICT screening method. No virus was detected in groups A and B, while three samples were positive in group C (1 boy and two girls), and 31 samples (23 boys and 8 girls) were positive in group D. The results of quantitative RT-PCR exclusively showed DEN-3 serotype in all the ICT positive samples. The results indicated that the prevalence of DEN-3 serotype in children was 100%, indicating that DEN-3 serotype might cause severe epidemics in the future in Lahore, Pakistan. Hematological analysis revealed an increase in hematocrits in 41.1% dengue-positive cases. Leucopenia was prominent in 79.4% of the cases, while Thrombocytopenia was reported in 70.5% of the participants. The biochemical analysis also indicated an increase in liver enzymes in patients (ALT 88%, AST 79%), while the lower levels of cholesterol (69 %) and serum albumin (25%) were also observed. Conclusions: Dengue virus spreads and grows quickly worldwide over a highly short time interval. Dengue fever claims for a significant number of lives. This study would help individuals know about the status of laboratory parameters in dengue fever and detect how to overcome the prevalence of Dengue virus.

Background: Mallotus Philippinensis belongs to the family Euphorbiaceae was broadly utilized in traditional medicines especially in the tropical areas. Mostly distributed in the Himalaya from Kashmir East wards besides tropical India. The plant contains high content of steroids, diterpenoids, triterpenoids, flavonoids, phenols, proteins, saponins, alkaloids and carbohydrates. The previous studies demonstrated that plant exerted significant pharmacological effects like anti-helminthics, antifilarial, antiparasitic, antiulcer, antifungal, antibacterial and immuno-regulatory properties, and as an aphrodisiac. Still antimicrobial activities from different solvent extracts of this plant needed to explore.Objective: The goal of the present investigation was to evaluate the phytochemical constituents, antibacterial, antifungal and antioxidant activity from the extract of plant Mallotus Philippinensis. Method: The ethanolic crude extract of plant was extracted by using maceration method. The resulting extracts were used to examined the antibacterial activity by disc diffusion method against some clinical strains (Bacillus, E. coli, S. aureus, Salmonella, Epidermites, E. facillus, Pseudomonas) and some ATCC strains (Bacillus, E. coli, S. aureus, K. pneumonia, Pseudomonas) and Phytoconstituents was also investigated for the presence or absence of various metabolites like proteins, carbohydrates, alkaloids , steroids flavonoids and saponin test. The DPPH method was used to determine the antioxidant activity. Results: The ethanol extract exhibit positive results for the inhibition of Bacillus, E. coli, S.aureus, Salmonella , Epidermites, E. facillus , Pseudomonas and also exhibit positive results for ATCC strains Bacillus , E.coli , S. aureus , K.pneumonia , Pseudomonas. The extract showed the presence of proteins, steroids, carbohydrates, alkaloids, flavonoids and saponin in the phytochemical screening. DPPH free radical scavenging activity assay disclosed the remarkable antioxidant activity of different parts of Mallotus Philippinensis ethanolic crude extracts. Conclusion: The findings of present project indicated that ethanolic extracts of Mallotus Philippinensis are sensitive against test bacteria. They also show antioxidant activity, and they contain rich number of phyto-constituents. In current study only crude extract was investigated for different curative effects against certain standard microbial strain. In future these extracts were considered after purification for development of various therapeutic agents.