Abstract BACKGROUND OF THE STUDY: Β-carotene, flavonoids and phenolic compounds found in methanol extracts of Moringa leaves and papaya fruit have high antioxidant activity so that they can be used for antiaging. The concentration of solvent is a factor that will affect the levels of active ingredients in methanol extract. This study aims to determine the effect of the concentration of the methanol solvent derived from the extraction of Moringa leaves and papaya fruit on the inhibitory activity of the elastase and hyaluronidase enzymes. AIM OF THE STUDY: Knowing the best extracts of methanol 50, 70, and 96% of Moringa leaf (Moringa oleifera) and papaya fruit (Carica papaya) as antiaging agents through inhibition of elastase and hyaluronidase enzymes. METHODOLOGY: In this study variations in the concentration of methanol 50%, 70%, and 96% were used for the extraction of Moringa leaves and papaya fruit. The extract was obtained by maceration method which was then tested for the inhibition of the enzyme activity of elastase and hyaluronidase using ELISA (Enzyme-Linked Immunosorbent Assay). RESULTS: The results of the elastase enzyme inhibitory activity test on Moringa leaf extract with 50%, 70%, and 96% methanol solvents IC50 respectively = 9453.38; 6604.70; 12346.44 μg / mL, while the yield of papaya extract 5995.31; 9046.25; 11571.54 μg / mL. In addition, the test results showed the inhibitory activity of the hyaluronidase enzyme showed that the Moringa leaf extract with 50%, 70%, and 96% solvents IC50 respectively = 2944.53; 1028,36; 3001.83 μg / mL, while the yield of methanol extract of papaya fruit is 982.67; 2982.96; 3530.18 μg / mL. CONCLUSION: Based on the test results it can be concluded that the most effective solvent concentration as an inhibition of the enzyme elastase and hyaluronidase methanolic extract of Moringa leaves is 70% methanol solvent. While papaya fruit methanol extract which is effective in inhibiting the enzymes elastase and hyaluronidase is 50% methanol solvent.

BACKGROUND: The bark of pulai (Alstonia scholaris [L] R.Br.) by the Gorontalo community is used for powder preparation (as a wedding ceremony custom), containing flavonoid compounds that function as sunscreens. Thus, the bark of pulai can be made in pharmaceutical preparations in the form of lotion dosage forms. AIM: This study aims to determine the SPF value of the lotion preparation of pulai bark extract. There are four formulas, formula F0 (lotion base), formula F1 (0.5%), formula F2 (1%), formula F3 (2%). METHODOLOGY: Physical evaluation of lotion preparations was carried out with organoleptic test parameters, spreadability, homogeneity, stability, pH. The SPF value of the lotion preparation was tested using the ultraviolet-Vis Spectrophotometry method with a wavelength of 290–400. RESULTS: The results of the physical evaluation of the organoleptic test and the homogeneity test were obtained that the lotion had a clear white color (F0), light yellow (F1), yellow (F2), dark yellow (F3), a characteristic smell of olive oil, and the four formulas had a thick and homogeneous consistency. The dispersion power of F0, F1, and F2 does not meet the requirements of the dispersion test value except F3. The three lotion formulations F1, F2, and F3 showed physical instability, except for F0 (lotion base). The pH of the lotions of the four formulas was in the range that was in accordance with the standard of topical preparations. CONCLUSION: The determination of the SPF value in the formulas F1, F2, and F3, respectively, was 3.3427 (minimum protection), 4.7752 (medium protection), and 5.0968 (medium protection).