The current investigation was executed at in a private the Orchard, located at El-Badary district, Assiut Governorate, Egypt, to study the effect of organic and bio-form fertilizers on vegetative growth, nutrient status and fruiting of Manfalouty pomegranate trees during 2019 and 2020 seasons. The experiment was arranged in a complete randomized block design with five replications and it consisted of five treatments.The results can be summarized as follow:Using the recommended dose of nitrogen (RND) via thrice form (mineral plus organic and bio-form) significantly increased the shoot length compared to use RND via mineral-N fertilizer (check treatment). All fertilization treatments significantly increased leaf area, chlorophyll and contents of N, P and K in leaves compared to check treatment. The fertilization treatments significantly increased the yield/tree and significantly decreased the fruit splitting and sunburn percentage compared to use mineral-N source only. Fertilization with any twice form (organic plus mineral)or thrice form (organic + bio + mineral) significantly improved the fruit properties by increasing the fruit weight, arils percentage and total soluble solids as well as sugar, anthocyanin and vitamin C contents and decreasing the total acidity compared to using the check treatment.It is clear from the current results the importance of fertilization through organic and bio-form for pomegranates productivity, via improve the fruit quality which reflects on induce the increase packable yield. In addition, such fertilization treatments reduce the cost of production and environmental pollution.