Colistin is considered as a last resort agent for treatment of severe infections caused by carbapenem-resistant Enterobacterales (CRE). Recently, plasmid-mediated colistin resistance genes (mcr type) have been reported, mainly corresponding to mcr-1 producers. Those mcr-1-positive Enterobacterales have been identified not only from human isolates, but also from food samples, from animal specimens and from environmental samples in various parts of the world. Our study focused on the occurrence and characterization of mcr-1-positive Enterobacterales recovered from retail raw chicken in Egypt. From the 345 retail chicken carcasses collected, a total of 20 samples allowed to recover mcr-1-positive isolates (Escherichia coli, n = 19; Citrobacter freundii, n = 1). No mcr-2- to mcr-10-positive isolate was identified from those samples. The colistin resistance trait was confirmed for all those 20 isolates with a positivity of the Rapid Polymyxin NP (Nordmann-Poirel) test. Minimum inhibitory concentrations (MICs) of colistin for all MCR-1-producing isolates ranged between 4 and 16 μg/mL. Noticeably, 9 out of the 20 mcr-1-positive isolates produced an extended-spectrum β-lactamase (ESBL), respectively producing CTX-M-9 (n = 2), CTX-M-14 (n = 4), CTX-M-15 (n = 2), and SHV-12 (n = 1). Noteworthy, the fosA4 gene encoding resistance to fosfomycin was found in a single mcr-1-positive E. coli isolate, in which both genes were located on different conjugative plasmids. The pulsed-field gel electrophoresis (PFGE) patterns were identified, corresponding to 10 different sequence types (STs), highlighting the genetic diversity of those different E. coli. Whole-genome sequencing revealed three major types of mcr-1-bearing plasmids, corresponding to IncI2, IncX4, and IncHI2 scaffolds. The occurrence of MCR-1-producing multidrug-resistant Enterobacterales in retail raw chicken is of great concern, considering the possibility of transmission to humans through the food chain.

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Effects of functional feed additives based on the fungus Fusarium.S and organic acids were studied. The research studied corporal parameters, the safety, growth and development of young turkeys and pigs. It was found that the Asido Bio-TCIT feed additive improves the physiological state of piglets and turkey poults, reduces feed conversion, increases live weight and profitability of meat production.

Учредитель и издатель: Founder and editor: ФГБОУ ВО «Казанская государственная академия ветеринарной медицины имени Н.Э. Баумана» (ФГБОУ ВО Казанская ГАВМ) FSBEI HE «Kazan Bauman State Academy of Veterinary Medicine»(FSBEI HE KSAVM) Печатается по решению редакционной коллегии Казанской государственной академии ветеринарной медицины им. Н.Э. Баумана от 5 Декабря 2019 г

Antibiotics widely used in poultry farms can have a harmful effect on meat consumers through chemical residues. Thus, it is important for the safety of customers to find a natural alternative. The purpose of this study was to evaluate the effectiveness of adding a poly-enzyme preparation “Universal”, containing amylosubtilin (1300 U/g), protosubtilin (67 U/g) and celloviridine (2000 U/g), on the productivity of broiler ducklings of the Cherry Valley breed. The results of research have shown that the addition of the “Universal” drug in the diet helped increase the meat productivity of broiler ducks. The use of the “Universal” drug contributed to the better development of internal organs (heart, liver and stomach) in broiler ducks. The improvement in duck weight and meat yield may be attributed to the fact that the feed protein is best consumed under the influence of protosubtilin, one of the “Universal.” It is understood that protosubtilin supplies digestible peptides and amino acids with hydrolysis of plant proteins from grain raw materials, enhances the gastrointestinal tract’s enzymatic history, compensates for the loss of depleted digestive enzymes and at the early stages of animal growth. Thus, the results of the experiment showed that the use of the drug “Universal” in the cultivation of broiler ducks of the cherry valley breed increases the pre-slaughter and slaughter weight, meat yield and reduces feed conversion, which is economically profitable. Therefore, it is recommended to use the drug “universal” as part of ration for broiler ducklings.