A case study of patient presented with developed wound infection with Pseudomonas aeruginosa (leukocytosis). The strains of P. aeruginosa isolated from Sheffield General Northern Hospital (UK) and studied for phenotypic and genotypic identifications. This present study were conducted in Sheffield Hallam University (UK) and was aimed to evaluate the application of Phenotypic and Genotypic techniques to identify P. aeruginosa. Generally, the results of Real time PCR (RTPCR) revealed that the diagnosis viability was confirmed for P. aeruginosa. However, identification on the basis of phenotype by differences in types of growth on cetrimide and acetamide agar medium presents that both agars were conducive to the growth of P. aeruginosa. The strains of P. aeruginosa were also tested for antibiotic susceptibility to six different antibiotics, imipenem showed the greatest inhibition effect on the bacteria. It was observed that genetic techniques in accordance with phenotypic tests have facilitated to conduct a comprehensive characterization of P. aeruginosa strains obtained from a particular environment at a particular time.