Fusarium wilt caused by Fusarium oxysporum (Fo) is the maximum critical soil borne diseases of chickpea in Egypt and many other countries in the world. Sixteen F. oxysporum (Fo) isolates were collected from 9 Governorates in Egypt isolated from wilted chickpea plants. Identification of these isolates was achieved by variation in morphological characters i.e., growth rate, growth habits, pigments, sporulation, and the morphological identifications were further confirmed by molecular method using specific ITS primers. Virulence of these isolates was classified as high virulence (Fo7, Fo8 and Fo10), moderate (Fo2,
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