Agro-morphological characters in 34 populations of Iranian Trifolium resupinatum L. germplasm, were investigated. 24 traits for each accession were evaluated according to white clover and forage legume descriptors.  Data were analyzed by SPSS and Excel softwares. Statistical tendency parameters for agro-morphological characters were calculated.  A cluster analysis based on morphological traits separate populations in two different groups that in one cluster there was an only accession belongs to Kurdestan locality.  Factor analysis have shown that 61/24% of collection variations were expressed by six factors. Leaflet shape, calyx trichome, the length of leaflet and inflorescence diameter were the most important morphological characters and the weight of 1000 seeds, number of days until to flowering were significant agricultural traits. . Based on these results, it seems that variation in germplasms is independent to geographical distribution. This research showed that there is a high biodiversity for this species in Iran due to the diverse environmental conditions, so that be useful for breeding programs and marketing.

The genus Vicia L. is an important forage crop and consists of approximately 160 annual and perennial species. This genus has variety in phenotypic characters. In this study, six genotypes of this taxon was choosen to determine the chromosome numbers, ploidy levels and to compare the karyotypic trairts. Based on the results, the basic chromosome number varied from x=5, x=6 and x=7. According to Stebbins classification, species were classified as symmetric class of 3A. Based on intra chromosomal symmetry, V. villosa Roth. had the most asymmetrical and evolutionary karyotype. V. cordata (Wulf. ex Hoppe) Arcangeli, Comp. had the most symmetrical karyotype. The highest distance was observed between these two species using cluster analysis.

Iran is considered one of the important centers of diversity of the Persian clover. In order to introduce the best populations for their marketing and sales, 48 accessions were collected from various parts of country and relationship between geographical distribution with ecological factors were studied. The results showed that the distribution of populations is mainly in the north, northwest and the center of country. Altitude and rainfall are two important environmental factors in the distribution of this species. Also it can grow on a wide range of soils but does better on fertile heavy moist clays. It is suggested that the accessions of Persian clover that are grow and cultivated in the north and northwest of the country are more important in marketing in terms of favorable geographical conditions, which should be considered by marketers.