Nano-structures of cobalt(II) metal-organic compound (Co-MOC) [Co(L)2(H2O)4] {1} (L = pyridine-3-carboxylic acid), have already been fabricated using two numerous experimental techniques which are including branched tube and sonochemical strategies. Besides the technique employed for 1, an identical crystal structure was obtained. Scanning electron microscopy (SEM), powder X-Ray diffraction (PXRD), as well as Fourier transform infrared spectroscopy (FT-IR), were applied for the characterization of all compounds. Single-crystal X-Ray diffraction (SCXRD) analyses of 1 reveal which Co2+ ions are 6-coordinated. Additionally, the temperature, time of reaction, the concentration of reactant, and sonication energy on the formation and ultimate morphology of compounds acquired by the sonochemical strategy are investigated. Ultimately, TGA (thermal gravimetric analyses), DTA (differential thermal analyses), as well as Hirshfeld surface analysis (HAS) of 1, were studied. Two strains of gram-negative Escherichi coli (E. coli) and gram-positive S. aureus) Staphylococcus aureus) was refined to the current compounds of 1 to determine their biological activities. These compounds exhibited adequate antibacterial activity within an abiotic environment.