BACKGROUND: Lempuyang, or widely known as wild ginger, has been used in Indonesia as traditional medicine for a long time. However, scientific research supporting its medicinal use is still very limited. AIM: This research was conducted to investigate the anxiolytic activity of three species of Indonesian Lempuyang (Zingiber zerumbet, Zingiber aromaticum, and Zingiber americans). METHODS: Sixty-six male Swiss Webster mice were divided into 11 groups (negative control, positive control, and experiment groups consisting of low, moderate, and high dose of Z. zerumbet, Z. aromaticum, and Z. americans extract, respectively). Anxiolytic activity was evaluated by three methods: elevated plus maze test, open field test, and hole board test. RESULTS: The results showed that these three species of Indonesian Lempuyang lowered the anxiety response in elevated plus maze test, open field test, and hole board test compared to negative control (p < 0.05). The best anxiolytic activity was achieved by moderate dose of Z. americans (8.5 mg/20 gbb), consistently through three methods applied. CONCLUSION: This research supports the potential use of the ethanolic extract of Lempuyang (Z. zerumbet, Z. aromaticum, and Z. americans) as a complementary therapy for anxiety.