The leaves of seventeen cultivars of olive growing in the north of Iran were investigated for total phenol content and antioxidant activity. The identification and quantification of main phenolic compounds were performed by reverse phase high performance liquid chromatography with diode array detector. The cultivars Kalamon, Gordal, and Coratina contained the highest concentration of phenolic compounds (190.65 ± 0.03, 184.72 ± 0.001, and 155.91 ± 0.06 mg GAE/g extract, respectively). The maximum radical scavenging activities were found in Gordal, Coratina, and Kalamon extracts (IC 50 20.66, 22.95, and 26.74 lg ml -1 , respectively). The extracts of Mishen, Fishomi, and Arbequina (1971.37 ± 0.007, 1794.57 ± 0.001, and 1760.57 ± 0.005 lmol Fe II/g dried extract, respectively) showed highest antioxidant activity in FRAP assay. The identification analysis demonstrated the present of vanillin, rutin, luteolin 7-O-glucoside, oleuropein, and quercetin.The highest oleuropein concentrations were detected in cultivars Mishen, Beleidi, Kalamon, and Roghani while it was not detected in cultivars Conservolea, Amigdalolia, Leccino, and Fishomi.

Clotrimazole is an antifungal agent, widely used in vulvovaginal and oropharyngeal candidiasis. Currently available clotrimazole (especially vaginal) dosage forms, have some limitations, including: leakage, messiness and low residence time, which leads to poor patient compliance. Therefore, in this study, a clotrimazole mucoadhesive gel has been developed as a suitable strategy due to their high water content, increased local retention time, lubrication and patient compliance. Xanthan gum and tragacanth with different portions were utilized as natural mucoadhesive gel forming polymers. Gel formulations were subjected to physico-chemical evaluations including gel viscosity, FTIR spectroscopy, spreadability, scanning electron microscopy (SEM) images of hydrogel chains, and release kinetic. Results demonstrated that among 8 developed formulas, formulation F1 showed the appropriate properties including controlled drug release (63.13% in 6h) with higuchi release kinetic, higher mucoadhesion (77.71 dyne cm-1) and drug content (94.47%) and relatively low spreadability (3.5 cm) which is suitable for local drug delivery. FTIR spectroscopy revealed there is not incompatibility between clotrimazole and other excipients in formulations. Combination of natural polysaccharides can form a proper mucoadhesive gel matrix for delivery of synthetic or natural drugs.