Amblyopia is a common visual impairment in developing countries and a major but neglected health issue. Definite diagnosis in children is difficult due to lower cooperation in this age range. Electroretinography (ERG) may be a useful objective method for diagnosis of amblyopia. In this study, we compare the ERG findings in amblyopic and non-amblyopic children. It is concluded that ERG is a sensitive and objective diagnostic test for amblyopia. It is recommended that ERG be used as a quick tool for diagnosis of amblyopia in children.