The objectives of the present study were to describe the current reproductive parameters of Holstein dairy cows in Iran and to determine factors which have a significant effect on reproductive performance with emphasis on high-producing dairy cows. Five large Holstein dairy herds from the major milk production areas of Iran were selected for this study. Reproduction data including parity of dam, calving date, days to first service, days open, number of services, dry period, and occurrence of diseases for cows that calved from 2004 to 2007 were collected from the herds' databases. To determine the effects of year and season of calving, disease status, level of milk production, and parity of dam on reproductive parameters, the proc-mixed procedure in SAS software was used. Overall, data about 8,204 lactation were collected for the study. Results showed that mean (+/-SD) days open, calving interval, and days to first service for study herds were 134 (+/-89), 403 (+/-86), and 67 (+/-38) days, respectively. Conception rates at the first service and the overall service-conception rate were 41.6% and 41%, respectively. The level of milk production and diseases had significant negative effects on days open and service per conception (P < 0.05); statistical analysis showed that for every 100-kg increase in milk yield, days open will increase by about 0.3 days. However, no significant effect of level of productivity was observed on days to first service. The results of this study indicate that high milk production is a risk factor for decreasing fertility in Iran, like many other parts of the world.