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The study aimed to isolate and purify the cationic Antimicrobial peptides (Amps) from the blood of sheep, bovine and camels, then study its antimicrobial activity against some multidrug resistant pathogenic bacteria (Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa , E.coli : 0157: H7), and compare it with some antibiotics. The results showed that those peptides could be obtained from various animals using the techniques in this study. The results of HPLC technique showed that there are (6) peaks in the crude

This research was performed to isolate and purify the Plant Antimicrobial peptides (Cyclotides type) from Rubiaceae family (Gardenia florida and Coffea arabica), and study their antimicrobial activity against some multidrug pathogenic bacteria (Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa, E.coli: 0157: H7), and compare it with theantibiotics effect. The results of isolating and purifying these Cyclotides showed that it could be obtained from the studied plants. The RP-HPLC technique results showed that there were (9) absorption peaks in