BackgroundCotton yield has been badly affected by different insects and weed competition. In Past Application of multiple chemicals is required to manage insects and weed control was achieved by different conventional means, such as hand weeding, crop rotation and polyculture, because no synthetic chemicals were available. The control methods shifted towards high input and target-oriented methods after the discovery of synthetic herbicide in the 1930s. To utilise the transgenic approach, cotton plants expressing the codon-optimised CEMB GTGene were produced in the present study.ResultsLocal cotton variety CEMB-02 containing Cry1Ac and Cry2A in single cassette was transformed by synthetic codon-optimised 5-enolpyruvylshikimate-3-phosphate synthase gene cloned into pCAMBIA 1301 vector under 35S promoter with Agrobacterium tumifaciens. Putative transgenic plants were screened in MS medium containing 120 µmol/L glyphosate. Integration and expression of the gene were evaluated by PCR from genomic DNA and ELISA from protein. A 1.4-kb PCR product for Glyphosate and 167-bp product for Cry2A were obtained by amplification through gene specific primers. Expression level of Glyphosate and Bt proteins in two transgenic lines were recorded to be 0.362, 0.325 µg/g leaf and 0.390, 0.300 µg/g leaf respectively. FISH analysis of transgenic lines demonstrates the presence of one and two copy no. of Cp4 EPSPS transgene respectively. Efficacy of the transgene Cp4 EPSPS was further evaluated by Glyphosate spray (41 %) assay at 1900 ml/acre and insect bioassay which shows 100 %mortality of insect feeding on transgenic lines as compared to control.ConclusionThe present study shows that the transgenic lines produced in this study were resistant not only to insects but also equally good against 1900 ml/acre field spray concentration of glyphosate.Electronic supplementary materialThe online version of this article (doi:10.1186/s13104-015-1397-0) contains supplementary material, which is available to authorized users.

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Ultimate tensile strength (UTS) becomes a primary concern in direct recycling of metal chips. This study investigates the influences of preheating temperature, preheating time and volume fraction of alumina on the tensile strength performance. The parameters of temperature, time and volume fraction of micro alumina were varied between 450 - 550 oC, 1 - 3 hours and 5 - 15% respectively. The full factorial design with center point analysis was employed to analyse the effect of process variables on the response. A total of 19 experimental runs were performed through the hot extrusion operation. The preheating temperature and volume fraction were identified as the key variables affecting the UTS. An optimum UTS was obtained for the profile extruded at 550 oC, 3 hours duration and 5% volume fraction of alumina.

A Zea may is an important cereal crop. To nourish human and livestock, it is very important that the quality of maize grain and fodder must be higher. A study was conducted to evaluate maize accessions for grain and fodder quality traits. Results indicated that higher heritability was found for nutrient detergent fiber, fodder cellulose, fodder crude fiber, fodder crude and fodder moisture percentage while genetic advance was higher for fodder cellulose, fodder crude protein and fodder ash percentage. High significant genotypic and phenotypic correlation was found among grain protein, oil and starch percentage, nutrient detergent fiber, fodder cellulose, fodder crude fiber & protein and fodder moisture percentage. The higher cumulative additive effect was recorded for acid detergent fiber, fodder crude fiber; nutrient detergent fiber and fodder cellulose suggested that selections may be made to develop synthetic varieties for better quality. Higher dominance effect and degree of dominance indicated that selection may be useful for the development of good quality maize hybrids through heterosis breeding programme. Principle component bi-plot analysis indicated that B-11×EV-347, B-11, Sh-139, EV-1097×E-322, Sh-139×B-316, B-327×E-322, B-316, Raka-poshi, B-11×Pop/209, B-336×EV-340, B-327×E-322, B-327×F-96, EV-1097×E-322, Rakaposhi×EV-347, EV-1097×Pop/209 and EV-1097×EV-340 performed better for grain and fodder quality and may be used for improvement of grain and fodder quality of maize.