Background Malaria is a protozoan disease which can lead to serious complications if not treated early and correctly. The aim of this study to assess the knowledge about malaria management guidelines among house officers. Methods this is the cross-sectional observational study conducted at 6 of Khartoum teaching hospitals Results The study showed that among the 115 participants evaluated there were 70.4% females and 29.6% were males. 94.8% of participants knew there are malaria management guidelines and 5.2% didn't know about the presence of these guidelines, 58.3% have some information and 10.4% just hear about these guidelines. 89.6% knew the management of simple malaria is outpatient management. 65.2% of the participants were aware of where to manage the cases of complicated malaria (inpatient or in ICU). 27.8% of the participants were aware of the management of simple malaria in the second and third trimester.