Objectives Enhance androstadienedione (Androst-1,4-diene-3,17-dione, ADD) production of rough-type morphotype Mycolicibacterium neoaurum variant by repeated-batch fermentation of immobilized cells. Results M. neoaurum R was a rough colony morphotype variant, obtained from routine plating of smooth M. neoaurum strain CICC 21097. M. neoaurum R showed rougher cell surface and aggregated in broth. The ADD production of M. neoaurum R was notably lower than that of M. neoaurum CICC 21097 during the free cell fermentation, but the yield gap could be erased after proper cell immobilization. Subsequently, repeated-batch fermentation by immobilized M. neoaurum R was performed to shorten the production cycle and enhance the bio-production efficiency of ADD. Through the optimization of the immobilization carriers and the solvents for phytosterols, the ADD productivity of M. neoaurum R immobilized by semi-expanded perlite reached 0.075 g/L/h during the repeated-batch fermentation for 40 days. Conclusions Although smooth strains that could homogenously suspended in broth seemed to be preferred in the steroid bioconversion, the rough-type strain M. neoaurum R might be able to find their place by proper cell immobilization.

Introduction: Biochemical analysis or laboratory tests assist to determine what happening to the body internally. The aim of the present study was to access the biochemical features of type 2 diabetic patients in Kushtia district, Bangladesh. Materials and Methods: The cross-sectional study was conducted in type 2 diabetes mellitus patients who presented to the OPD in three referral diabetic centers in Kushtia district, Bangladesh from August 2016 to July 2017. A total of 282 patients were included in the study. The collected data were checked, entered and analyzed using the computer program Statistical Package for Social Sciences (SPSS) version 22. Results: Biochemical study showed that mean blood hemoglobin level was 12.49±1.47g/dl, mean fasting blood glucose level of the patients was 8.86±1.67mmol/L and blood glucose level two hours after breakfast was 13.94±2.88mmol/L. The study showed that the patient having abnormal level of triglyceride (66%) were higher than normal level (34%). Conclusion: The subjects had higher triglyceride and LDL values (65.6% and 28.7% respectively). These higher percentages with abnormal lipid profile are likely to be more prone to cardiovascular diseases. Medicine Today 2021 Vol.33(1): 75-79