The model showed adequate validity to be applied in a large-scale production of both lysine and glutamic acids.

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Keywords: Amino acids production; Mucor mucedo strain SEE1; protease; statistical optimization; 18S rRNA

Five fungal strains namely Aspergillus niger 6A, A. niger NRRL, A. niger NRC, A. fumigatus and A. oryzae were used in this study. Five different plant materials which underground roots and tubers were directly used in powder form as natural sources. They were chicory (Cichorium intibus), dahlia (Dahlia pinnata), Girasole or jurusalem artichoke (Helianthus tuberosus), suger beet (Beta vulgaris) and artichoke or Alkhrishv (Cynara cardunculus). Dahlia tubers and Girasole additions were the best inducer for inulinase production amongst other plant materials used. On the other hand, chicory, alkhrishv and sugar beet additions had negligible and/or negative effects on inulinase production by the tested fungal srtains.

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