To evaluate the fracture resistance of endodontically treated mandibular premolars restored with glass fiber posts using different luting agents. Methods: Twenty-four extracted single-rooted mandibular premolars were endodontically treated, and post spaces were prepared to receive fiber posts. They were assigned to three test groups (n=8) according to the type of cement used for the cementation of glass fiber posts: RC group: adhesive resin cement group (etch and rinse), SC group: self-adhesive resin cement group, and GC group: glass ionomer cement group. Teeth in all groups were adhesively restored with a composite resin core material and crowned with Ni-Cr crowns. All specimens were subjected to tangential loading using a universal testing machine until fracture at 30°. Failure loads were recorded, and data were analyzed using one-way ANOVA followed by Tukey's HSD test (α=0.05). Results: Specimens in the RC group were more resistant (258.3±12.7 N) to fracture than those in the SC (218.7±11.1 N) and GC (165.4±8.9 N) groups (P≤0.001). One-way ANOVA indicated that the type of cement had a significant effect on the fracture resistance of endodontically treated lower premolars (P≤0.001). Conclusion: The type of cement that was used to fix glass fiber posts was a determining factor of the fracture resistance of endodontically treated lower premolars.