Introduction: Carcinoma breast is one of the commonest causes of death in many developed countries in women. Cancer that is detected at an early stage can potentially be cured when the tumor is small enough to be completely removed surgically. Keeping this in view of these phenomena, the present study takes into consideration of certain biochemical investigations like Serum LDH, GGT and ALP levels that are inexpensive, accurate, and identified by the easy method of detection, and to establish their establish their diagnostic and prognostic value in breast cancer Patients and Methods: The present study comprises of 120 participants of which 60 healthy controls, and 60 patients suffering from carcinoma breast, which was confirmed by clinical and histopathological examination. Results: Serum LDH ,GGT and ALP levels are significantly increased in breast cancer patients compared to controls and also found that the levels was gradually increased with advancing stages of breast cancer. Conclusion:It is concluded that serum LDH, GGT and ALP levels are reliable and inexpensive promising enzyme markers with prognostic and diagnostic importance in carcinoma breast patients.