BackgroundLeptin is a neuroendocrine protein hormone with 167 amino acids mainly produced by adipose tissue that regulates food intake and energy expenditure to maintain relative stability of body weight and energy storage over long period of time. A single nucleotide polymorphism in leptin, consisting of G to A substitution at nucleotide −2548 upstream from ATG start site (LEP G‐2548A, rs7799039) has been found to influence leptin expression, possibly at the transcriptional level and was associated with increased leptin secretion in adipocytes. However, these high levels of leptin do not serve their function in controlling the obesity of these individuals possibly due to a condition known as ‘leptin resistance’.AimAssess the risk of the genetic polymorphism of leptin gene G‐2548A on obesity.Material and MethodA case‐control study which consisted of 66 individuals of different BMI (46 normal, 16 overweight and 4 obese). The different genotypes of leptin G‐2548A were determined by restriction fragment length polymorphism polymerase reaction after DNA extraction from saliva samples.ResultsA high risk of obesity was seen among carrier of AA genotype compared to other genotypes (GG&GA) (OR=2.6, 95% CI= 0.9–7.5). Furthermore AA genotype was found to be a risk factor for abdominal obesity (OR=1.3, 95%CI= 0.5–3.7).ConclusionCarrier of AA genotype of leptin G‐2548A polymorphism are at risk of obesity.Support or Funding InformationRak Medical and Health Sciences UniversityThis abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.