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In this article, the effect of soil properties and earthquakes on a structure built in a multi-layer foundation soil is studied using Abaqus/СAЕ. Single foundations are one of the most common types of foundations traditionally required to support buildings in earthquake-prone regions. Most researchers work on the dynamic interaction between the foundation and the soil, which can affect the response of the building during earthquakes due to the type of earthquake and the properties of the soil layer. In this paper, four different earthquakes were applied to a reinforced concrete building constructed in two-layer soils with very different soil properties, and a discussion on soil-structure interaction was opened using Abaqus software. In the analysis, a layered soil is simulated with 4 different models (soft-hard, hard-soft). Through these analyses, the influence of the different soil layers (soft and hard), the building displacement and the frequency content of the earthquake were studied. According to the result of the analysis, it is important to consider the earthquake characteristics, the soil properties, and the type of foundation in the dynamic soil-structure interaction.