Assurance of analysts of various matrices (for example natural or tainted water or soils) at a standard level are often required for identifying the environmental pollution. A couple of used systematic methods are delicate enough for the direct assurance of trace components in samples, therefore, a preliminary step of the analyst prior to analysis is consuming much of the time. One of the best alternative methods that uses surfactant to extract organic/inorganic parts is the cloud point extraction CPE. It is a fast, easy and economical extraction technique includes assembly of monomers of nonionic surfactant to form a hydrophobic core (micelle). At that point, the micelles entangle the hydrophobic bioactive mixes inside the micellar extraction. This field of extraction is very promising for new and active analytical strategies that are applicable to many systems. This review shows the importance of CPE method, surfactants, procedures used to detect the cloud point, the effect of different parameters on the efficiency of extraction and the possible applications and limitations of the method. It aims to explore the possibility of developing CPE within the approach of green analytical chemistry.

In this study, new spectrophotometric methods development for evaluation of ciprofloxacin are described. The first method including conversion of ciprofloxacin to colored complex with Fe (II) in the alkaline medium. The colored product has a yellow color with absorbance at λmax 450 nm. Between the concentration range (2-90 μg.mL−1), the Beers law is obeyed with correlation coefficient (R2) as 0.9996, limit of detection as 0.365 μg.mL−1, limit of quantification as 1.189 μg.mL−1. Secondly, cloud point extraction was used to determine of a trace amount of the colored product in the first method followed by measuring with a UV-Vis spectrophotometer. The linearity of calibration curve was above the range of (1-50μg.mL−1), the correlation coefficient (R2) was 0.9995. The Limit of detection (LOD) and Limit of quantification (LOQ) were found to be 0.129 and 0.387 μg.mL1 respectively. This method was successfully employed for ciprofloxacin detection in the several pharmaceutical samples by Rec. Was rang between (98-102.8).

Ponceau 4R(E124) is azo dye that is mostly used in some of food products such as soft drinks, beverages, jelly and ham. A simple and sensitive procedure is established for the determination of ponceau 4R using cloud point extraction (CPE). In order to get the optimum conditions for ponceau 4R extraction, various analytical factors such as pH media, nonionic surfactant TX-114 or Brij-56, cationic surfactant CPC and equilibration temperature was investigated. The linearity of calibration graph was above the range of 0.05-1.7 µg ml-1. The LOD and LOQ using TX-114 were based to be 0.025, 0.075 µg ml-1 whereas for Brij-56 were found as 0.062, 0.187 µg ml-1 respectively. The proposed procedure was successfully employed for ponceau 4R detection in several beverages samples.

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In this work, new spectrophotometric techniques development for assessment of dexamethasone are described. The first technique including conversion of dexamethasone to colored compound with 2, 4-DNPH as reagent in the acidic medium. The colored compound has a yellow color with absorbance at 405 nm. Between the range of concentration (5.0-40 mg.L -1 ) , The beer , s law is obeyed with correlation coefficient as ( 0.9959), LOD as 0.507 mg.L -1 , LOQ as 1.5 mg.L -1 . The second procedure, in technique accompanied by measurement with a UV-Visible spectrophotometer, the CPE technique was used to determine the quantity of the color compound. The linearity of calibration curve between range of (0.5-6.0 mg. L -1 ), R 2 was 0.9974. LOD and LOQ were found to be 0.108 and 0.354 mg.L -1 respectively. This technique was successfully utilized for dexamethasone detection in the several pharmaceutical formulations by REC% was rang between (98-101).