Organizational culture plays a pivotal role in the development and change of organizations. To achieve institutional competitiveness and repositioning on the world market all key players in the institutional development need to be on the same page in terms of organizational culture. A mammoth of studies have been done in the past to explore organizational culture structure but there were no attempts done to validate and measure the construct on employee behaviour and thoughts at the Islamic University in Uganda. The purpose of this study therefore is to measure and validate organisational construct as perceived by staff at the Islamic University in Uganda. The study employed four dimensions to examine organizational culture, and 361 staff through randomization participated in the study. To arrive at the intention of the study SEM-Amos technique of data analysis was used to confirm the hypothesized measurement model. The results indicated that meaningful value, support and promotion of values, discipline values and free style value are true and valid predictors of organizational culture structure.