Background:Woodfordia fruticosa, a plant of Indian origin, is extensively used in folk medicine for the treatment of various ailments.Objective:The aim of the present study was to standardize the flowers of W. fruticosa, Kurz (Lythraceae), an important plant of Indian origin and explore the chemical constituents contributing to its anti-ulcer activity.Materials and Methods:High-performance thin layer chromatography (HPTLC) and high-performance liquid chromatography (HPLC) profiling of the three samples of W. fruticosa flowers purchased from three different markets was done using ellagic acid as the biomarker. Two doses of the aqueous extract of the W. fruticosa (AEWF) flowers were evaluated for anti-ulcer activity by ethanol-induced ulcer model in Wistar albino rats. Omeprazole was used as the positive control. The parameters used for the assessment of the anti-ulcer potential were total titratable acidity (TTA), ulcer index, and percentage protection.Results:The HPTLC and HPLC studies confirmed the presence of ellagic acid in all the three drug samples. The AEWF showed significant reduction in terms of TTA at both doses of 100 mg/kg and 200 mg/kg. The gastroprotection indicated by a lower ulcer index and higher percentage protection was significant for 200 mg/kg dose of AEWF, better than the protection afforded by omeprazole (10 mg/kg).Conclusion:The chromatographic profiling and the anti-ulcer studies served as an efficient tool in the characterization of ellagic acid as an important biomarker for the flowers of W. fruticosa and a probable contributor to the gastroprotective capacity of the drug. The bioactivity studies further supported the traditional use of W. fruticosa in the treatment of ulcers.SUMMARY HPTLC & HPLC fingerprinting of W. fruticosa using ellagic acid as a biomarker.Evaluation of W. fruticosa for gastroprotection potential in ethanol induced gastric ulcer in rats model.Aqueous extract of the drug showed better gastroprotection than the standard drug omeprazole at a dose of 200 mg/kg.

Objective: To assess the antidiabetic and hypolipidemic properties of Stereospermum colais Fruit. Methods: Acute toxicity test was done to check the toxicity of Stereospermum colais fruit methanol extract at three dose levels was administered orally to streptozotocin (STZ) (40 mg/kg bw) induced diabetic rats for 15 days. The various parameters were studied including body weight, fasting blood glucose levels, plasma insulin, lipid profile, glycogen content, glycoslylated hemoglobin (HbA1c) and serum marker enzymes levels in normal, treated and diabetic rats. Histochemical analysis of pancreas was also carried out in normal treated and diabetic rats. Results: The treatment group with the extract at three dose levels showed a significant increase in the liver, muscle glycogen and serum insulin level and a significant decrease in fasting blood glucose, glycosylated hemoglobin levels and serum marker enzyme levels. The total cholesterol and serum triglycerides levels were also significantly reduced and the high density lipoprotein level was significantly increased upon treatment with the Stereospermum colais Fruit methanol extract. Histochemical study of pancreas also confirmed the biochemical findings. Acute toxicity studies revealed the non-toxic nature of the Stereospermum colais Fruit methanol extract. Conclusions: The results of the experiments presented here suggest that methanol extract of Stereospermum colais Fruit exerts significant antidiabetic and hypolipidaemic effect in STZ induced diabetic rats.