Conservation agriculture has been proposed as an alternative to conventional agriculture to mitigate the climate change impact and ensure food security. This study examined the effect of three tillage systems, ((conventional tillage (CT), chisel tillage (ChT) and no tillage (NT)) and two crop-rotation systems ((two year crop rotation (2-yr) and three year crop rotation (3-yr)) on some yield and quality parameters of two winter durum wheat genotypes. The results showed that biological yield (BY) and thousand kernel weight (TKW) were neither significantly affected by tillage, crop rotation nor genotype. Only the genotype significantly affected grain yield (GY). Grain protein content (GPC %) showed higher values than straw protein content (SPC %) and NT negatively affected GPC%. Some significant differences of mineral elements were observed according to tillage system applied and NT showed the lowest values of grain N, straw K, straw Ca and straw Na. Tillage system, croprotation system and genotype had no effect on total phenolic content (TPC) and total flavonoid content (TFC). Highly significant negative correlation (-0,378**) was recorded between GY and grain N, and accordingly GPC%. The two winter durum wheat genotypes demonstrated that with the two crop-rotation systems, yields and most of quality parameters under NT and ChT were similar to those under CT. This study expands our knowledge on durum wheat chemistry variation in relation to agricultural system adopted and provides a basis for selecting the adequate crop