Objective: The objective of this study was to improve certain sperm function parameters in vitro of oligozoospermic patients and other mild male factors using sperm activation techniques such as swim-up and density gradient. Methodology: The study was carried out between January 2014 and October 2014 including (30) semen samples of oligozoospermic patients who attended the Fertility Center in AL- Sadr Medical City. The liquefied semen was then carefully mixed for few second and the semen was analyzed by macroscopic and microscopic examination. Two method of IUI sperm activation have been used in this study to separate the motile spermatozoa in seminal plasma. Data analyzed using the SPSS version(20) Results: The results of the study showed that there was statistical significant ( p<0.05) decrease in the sperm concentration, leukocyte concentration, malondialdehyde(MDA) concentration and sperm chromatin maturity after activation by both swim-up and density gradient techniques compared with before activation. Also the results was appeared a significant (p<0.05) improvement in progressive motility (A+B) and normal morphology after activation by both techniques while, there was a significant (p<0.05) improvement in progressive motility (A+B) was noticed by using density gradient technique compared to swim-up technique. Conclusions: It was concluded that the density gradient and swim-up techniques were appeared clear improvement in the certain sperm parameters of oligozoospermic patients after activation. Recommendation: The density gradient techniques with intrauterine insemination to treatment Oligozoospermic patients.

Background: Effect of seasonal variations in human fertility has been intensively researched; some studies acknowledge influences of seasonal changes on natural conception while others cannot confirm them. The aim of this study was to assess the presence of a possible Seasonal pattern in the functional parameters of Semen samples.Materials and Methods: This study was carried out on 335 Semen samples that were collected and analyzed according to the average highest temperature of the Normo-spermic men between January 2019 and December 2 014 at the Fertility Center in Al- Sadr medical city. Najaf Iraq.Results: ALL Semen Parameters displayed trends opposite to average highest temperature variations. Semen volume, sperm concentration sperm motility and normal sperm morphology were significantly lower (p<0.05) in summer than other seasons of the red year: Semen volume sperm concentra and normal sperm morphology was found to be statistically significantly higher in the winter (p<0.05). The percentage of sperm motility was a significantly (p<0.05) is higher in spring The highest values of Semen volume sperm concentration and sperm morphology was recorded in January and February, while a higher sperm motility was found during the march any of the year.Conclusion: We observed that was significant effect of season on the semen parameters. Highest environment temperature may be a determining factor for the seasonal variations in semen quality.