Problem statement in the context of performance sports, along with normal physiological reactions, there is competitive stress, which varies in relation to the type of sport practiced. Purpose of study: to test sports subjects at the beginning and end of the application of the intervention program. To test the biochemical and physiological condition of athletes and to modulate the reactivity of athletes to the stress of competition. Methods we proposed a training scheme that includes assessing the hormonal status of athletes by non-invasive methods, such as salivary and urinary determination. Research designs: the subjects of the study were juniors aged 14-16 from sports clubs in Satu-Mare County and Cluj-Napoca. Sampling: were urine and saliva samples were taken before and after training. These were in average volumes of 1 ml. Findings and Results: the statistics were processed with GraphPad Prism 5 software, with the student t test for unmatched values associated with Welch's correction. For biochemical markers that have been dosed the statistical significance is p <0.05 with a probability > 95%. Discussion: biochemical parameters for saliva and urine were assayed by immunochemical and spectrophotometric methods. It has been shown that specific training in this case contributes to the biochemical reaction of adaptation to stress (coping). Conclusions this approach is applicable in planning pre-competitive training to target the level of positive and negative emotions (functional and dysfunctional) in association with a level of stress.