According to recent studies, Chlamydophila pneumoniae has been associated with atherosclerosis and ischemic stroke. Proper treatment of C. pneumoniae can cause great improvement in public health. This study aimed to detect antibodies against C. Pneumoniae by ELISA in patients with ischemic stroke who were admitted to Rajaei and Imam -Sajad hospitals during 2012. In this case-control study, we compared the serum samples of 30 patients with ischemic stroke, confirmed with physical examination and brain computed tomography, as case group, with 30 age-and sex-matched patients of surgery ward as control group. Then serologic test was performed by ELISA. After admission of suspicious cases, we had 23 males (44.2%) and 29 females (55.8%) with 27 individuals in case and 23 in control groups. We found association between positive titers of IgG and IgA antibody against C. pneumoniae and ischemic stroke. There was just a positive association between IgG antibody titer and age in case group. Although we could not find any association between positive titers of IgG and IgA against C. pneumoniae and ischemic stroke, a significant association between IgG antibody titer and ages was confirmed (p<0.029).